OLIS DSM 20 CD用于测量单分子电导检测和鉴定遗传物质
Detection and identification of genetic material via single-molecule conductance
RNA模式的持续发现（例如，非编码，微观和增强子）导致对用于食品安全，水和环境保护，植物和动物病理学，临床诊断和研究，以及生物安全的RNA片段的检测，测序和鉴定的需求增加。在这里，我们证明单分子电导技术可用于从短RNA寡核苷酸中提取生物学相关信息，这些测量对阿托摩尔目标浓度敏感，它们能够被多重化，并且它们可以检测感兴趣的目标。存在其他可能干扰的RNA序列。我们还证明了RNA：DNA杂交体的电荷传递特性对单核苷酸多态性敏感，因此能够区分大肠杆菌的特定血清型。使用光谱和计算方法的组合，我们确定电导灵敏度主要来自突变对分子的构象结构的影响，而不是来自直接化学取代。我们相信可以进一步开发这种方法以制造用于诊断目的的基于电的传感器。
The ongoing discoveries of RNA modalities (for example, non-coding, micro and enhancer) have resulted in an increased desire for detecting, sequencing and identifying RNA segments for applications in food safety, water and environmental protection, plant and animal pathology, clinical diagnosis and research, and bio-security. Here, we demonstrate that single-molecule conductance techniques can be used to extract biologically relevant information from short RNA oligonucleotides, that these measurements are sensitive to attomolar target concentrations, that they are capable of being multiplexed, and that they can detect targets of interest in the presence of other, possibly interfering, RNA sequences. We also demonstrate that the charge transport properties of RNA:DNA hybrids are sensitive to single-nucleotide polymorphisms, thus enabling differentiation between specific serotypes of Escherichia coli. Using a combination of spectroscopic and computational approaches, we determine that the conductance sensitivity primarily arises from the effects that the mutations have on the conformational structure of the molecules, rather than from the direct chemical substitutions. We believe that this approach can be further developed to make an electrically based sensor for diagnostic purposes.
https://www.nature.com/articles/s41565-018-0285-x
