Zahra Gheshlaghi, Reza Khorassani, Javier Abadía, Ana Alvarez-Fernández, Adrián Luis-Villarroya, Amir Fotovat & Mohammad Kafi
Background and aims
The effects of root glutathione (GSH) supplementation on leaf chlorophyll, Fe concentrations and contents in leaves, stems and roots, and traits associated to Fe deficiency were studied in Medicago scutellata plants grown in rock sand under conditions of Fe deficiency, in the presence of different concentrations of bicarbonate.
Plants were grown in acid-washed rock sand irrigated with a zero Fe solution (pH 7.8 with 0.5 g L−1 CaCO3) or a 45 μM Fe(III)-EDDHA solution (5 mM MES, pH 5.5), with 0, 5 or 15 mM NaHCO3, and 250 mL of 1 mM GSH was added daily to half of the pots.
植物在酸洗过的岩砂中生长，用零铁溶液（pH 7.8和0.5 g L -1 CaCO 3）或45μMFe（III）-EDDHA溶液（5 mM MES，pH 5.5）灌溉，每天将5或15 mM NaHCO 3和250 mL 1 mM GSH添加到一半的锅中。
Iron deficiency caused characteristic symptoms in plants, with GSH supplementation relieving them. Glutathione supplementation led to increases in total Fe, chlorophyll and leaf total and extractable Fe, whereas root Fe concentrations decreased. Traits associated to Fe deficiency, including changes in biomass, root morphology, carboxylate contents and antioxidant parameters became less intense with GSH supplementation.
Glutathione supplementation allowed plants to take up Fe from the rock sand via a reductive solubilization mechanism. Also, the distribution of Fe within the plant changed, with more Fe being allocated to the shoot tissues and less to the roots.