补充谷胱甘肽可防止在碳酸氢盐含量不同的岩砂中生长的黄ca中的铁缺乏
Zahra Gheshlaghi, Reza Khorassani, Javier Abadía, Ana Alvarez-Fernández, Adrián Luis-Villarroya, Amir Fotovat & Mohammad Kafi
这篇论文用到的光谱仪就是Olis的CLARiTY
Abstract
Background and aims
The effects of root glutathione (GSH) supplementation on leaf chlorophyll, Fe concentrations and contents in leaves, stems and roots, and traits associated to Fe deficiency were studied in Medicago scutellata plants grown in rock sand under conditions of Fe deficiency, in the presence of different concentrations of bicarbonate.
索引
背景和目标
研究了补充谷胱甘肽(GSH)对在缺铁条件下在缺铁条件下在岩砂中生长的黄Medi药草中叶黄素,叶,茎和根中铁的浓度和含量以及与铁缺乏相关性状的影响。不同浓度的碳酸氢盐。
Methods
Plants were grown in acid-washed rock sand irrigated with a zero Fe solution (pH 7.8 with 0.5 g L−1 CaCO3) or a 45 μM Fe(III)-EDDHA solution (5 mM MES, pH 5.5), with 0, 5 or 15 mM NaHCO3, and 250 mL of 1 mM GSH was added daily to half of the pots.
方法
植物在酸洗过的岩砂中生长,用零铁溶液(pH 7.8和0.5 g L -1 CaCO 3)或45μMFe(III)-EDDHA溶液(5 mM MES,pH 5.5)灌溉,每天将5或15 mM NaHCO 3和250 mL 1 mM GSH添加到一半的锅中。
Results
Iron deficiency caused characteristic symptoms in plants, with GSH supplementation relieving them. Glutathione supplementation led to increases in total Fe, chlorophyll and leaf total and extractable Fe, whereas root Fe concentrations decreased. Traits associated to Fe deficiency, including changes in biomass, root morphology, carboxylate contents and antioxidant parameters became less intense with GSH supplementation.
结果
缺铁会引起植物特征性症状,补充GSH可以缓解这些症状。补充谷胱甘肽导致总铁,叶绿素和叶片总铁和可提取铁的增加,而根铁的浓度下降。补充谷胱甘肽与铁缺乏相关的性状,包括生物量,根系形态,羧酸盐含量和抗氧化剂参数的变化,变得不那么强烈。
Conclusions
Glutathione supplementation allowed plants to take up Fe from the rock sand via a reductive solubilization mechanism. Also, the distribution of Fe within the plant changed, with more Fe being allocated to the shoot tissues and less to the roots.
结论
谷胱甘肽的补充使植物通过还原增溶机理从岩砂中吸收铁。同样,植物中铁的分布发生了变化,更多的铁分配给了芽组织,而更少的铁分配给了根。