What is Stopped Flow?<\/strong><\/p>\n<\/div>\n<\/div>\n

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Absorbance, fluorescence, and circular dichroism are useful tools for monitoring kinetic changes of chemical and biological systems. However, changes often occur\u00a0on the millisecond scale, too fast for simple mixing. A stopped-flow accessory is used on the spectrophotometer to achieve fast mixing.Reagents are injected from two drive syringes through a mixer into the observation cell, where the changes in absorbance, fluorescence, or circular dichroism are recorded. Reactions ranging\u00a0from a few milliseconds to hundreds of seconds can be studied with the stopped-flow method.<\/p>\n<\/div>\n<\/div>\n
Stopped Flow Applications<\/span><\/strong><\/div>\n
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Typical research areas include reaction mechanisms, drug-binding processes, and determination of protein structure. Other applications include<\/p>\n
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Ligand binding<\/li>\n
Coordination reactions<\/li>\n
Chemical reactions<\/li>\n
Electron transfer<\/li>\n
Fluorescence resonance energy transfer (FRET)<\/li>\n
Protein folding<\/li>\n
Enzyme reactions<\/li>\n
Protein-protein interactions<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
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