Imagine getting exactly the right absorbance reading on suspensions as widely disparate in turbidity as the five samples shown.

The polystyrene particles used to create these suspensions have zero absorbance. Would your spectrophotometer return 0.0 AU as the answer for all five?

No. For decades, reliable, accurate absorbance studies have been dependent upon thehomogeneity and clarity of the sample. Turbid samples produce apparent high absorbances, not the correct answer.

For decades, spectrophotometrically working with turbid suspensions has proved elusive, because themeasurement beam is scattered in all directions; only a fraction of it passes through the sample to the detector. “Apparent high absorbance” is the result, as these examples show.

Scatter Does Not Matter! Only Sample Absorbance Does.

The CLARiTY allows you to rethink your possibilities.

Results from four popular and respected spectrophotometers compared with the Olis CLARiTY 1000

The sample was the most concentrated suspension of polystyrene particles in water (about 108 of the 1 micron particles/mL H2O) as shown on page 2. Recall, polystyrene particles do not absorb light, so the correct answer is 0.0 AU.

The CLARiTY gets zero absorbance, give or take 0.01 AU. As these and other data will show, scatter does not matter to the CLARiTY,including the UV, where even the “goldstandard” cannot handle the scatter correctly.


For bioenergetics & drug discovery breakthroughs.
Subsecond spectral acquisition rates, nanomolar sensitivity, and complete immunity to scatter.

The “fondest dream” for investigators working on enzyme kinetics, protein expression, blood research, microbe metabolism, algae photosynthesis, and more. Easily repurposed for stopped-flow spectrophotometry.


Technical Specifications:

  • Collect ‘spectral videos’ at rates to 100 scans/ second
  • When rapid-scanning, scans are 38, 77, 154, or 231 nm wide with corresponding spectral resolutions of 0.3, 0.6, 1.2, or 1.8 nm
  • When not rapid-scanning, scans can be of arbitrary length and selected spectral resolution
  • True sample and reference (dual beam) acquisition with two photomultiplier tubes
  • Spectral Range = 240 - 800 nm
  • Detection limit of 5 x 10-5 AU
  • Reconfigurable to the OLIS DSM 1000 CD and the OLIS RSM 1000 for stopped-flow and flash photolysis studies
  • Footprint 60" x 20" x 14"

Available enhancements include fluorescence detection, oxygen monitoring, actinic source, computerized shutter, ion electrodes, novel DSPCs, automatic titrator, and stopped-flow.

Related posts