【论文】FE-SEM characterization of neural cells with Vac Coat Sputter and Carbon coater

In a recent study, the role of the Indian herb Bacopa monnieri in treating oxidative stress in neuronal cells has been investigated through electron microscopy imaging. This study suggests that Bacopa monnieri and its active compounds could be used as a new drug for the prevention and treatment of oxidative stress-related neurodegenerative diseases as a natural remedy.

This article has investigated the therapeutic potential and molecular complexities of Bacopa monnieri, recognized for its neuroprotective and cognitive advantages, in treating neurological disorders and brain damage influenced by oxidative stress. The morphological features of the cells in response to the methanolic extract of Bacopa monnieri (BME) and its active component, Bacoside-A, checked through FE-SEM characterization, show enhanced neuronal cell protection against oxidative stress as in neurodegenerative diseases. The neuronal cells grown over coverslips underwent the required treatments and then were mounted on carbon tape which was pasted on the sample holder stage and prepared for observation after gold coating with Vac Coat sputter and carbon coater, DSCR. The SEM observation was carried out using a Zeiss high-resolution microscope.

SEM images (Scale bar: 20 μm) showing the morphology of HT-22 cells pre-treated with BME and Bacoside-A in presence of oxidative stress. The below figures show the morphology of neural HT-22 cells pre-treated with BME in contrast to the non-treated cells in the presence of oxidative stress (H₂O₂ treatment), comparable to the control cells (Yellow arrows show the damaged membrane by oxidative stress). Maximum protection was obtained in both BME (100 µg/ml) and Bacoside-A treated cells, nearly the same as the control cells.

SEM images (Scale bar: 20 μm) showing the morphology of HT-22 cells pre-treated with BME and Bacoside-A in presence of oxidative stress. The below figures show the morphology of neural HT-22 cells pre-treated with BME in contrast to the non-treated cells in the presence of oxidative stress (H₂O₂ treatment), comparable to the control cells (Yellow arrows show the damaged membrane by oxidative stress). Maximum protection was obtained in both BME (100 µg/ml) and Bacoside-A treated cells, nearly the same as the control cells.

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