Olis, Inc OLIS CLARiTY 245 Spectrophotometers 扫描荧光和吸收光谱仪
Research quality scanning spectrofluorimeter with one OLIS double grating f/4.2 Hummingbird monochromator on the excitation side and one OLIS single grating "sister" monochromator on the emission side. Excitation source is a water-cooled 150 watt xenon arc lamp run within a mirrored elliptical lamp housing designed for maximum intensity. The lamp is ignited and run with a high precision regulated power supply for highly reliable lamp starting, ultralow ripple, and high precision current regulation. Computerized emission and excitation wavelength selection is supported, with software settable scanning and signal averaging. Detection rates are up to 10 milliseconds, with an arbitrary amount of signal averaging during or after collection to optimize for speed or best sensitivity. One digital photon counting module (PCM) is provided for exquisite sensitive emission readings from 230-870 nm. A second detector, a high precision analog photomultiplier tube, is used for the absorbance readings, mounted directly adjacent to the sample.
The sample compartment is a single beam CLARiTY Chamber. The "CLARiTY" chamber eliminates the effect of scatter and thus allows accurate absorbance and fluorescence spectroscopy to be done on turbid sample suspensions. Within the "CLARiTY" chamber is the 'suspension presentation cavities,' (DSPC) which is either a high-absorbance (up to 22 AU) micro cuvette, or a 1mL, or 8 mL (low absorbance, to nanomolar concentration) quartz sample holder . The DSPCs return effective pathlengths of 0.25 millimeters, 5 centimeters, or 30 centimeters respectively. A large DSPC is best when sample volume is of no concern, as when it is E.coli, yeast, algae, and all other plentiful and/or inexpensive samples are used. The benefit of the large volume is the tremendous pathlength enhancement of approximately 30 cm. The small DSPC allows one to work with finite sample volume (e.g., embryonic zebra fish or infant blood cells). The pathlength enhancement is far less, only 4 centimeters for a 1 mL volume. But, one is flooding the smaller volume with the same light intensity, so there is a stronger detected signal and thus superior S/N. Ports holding quartz rods are built-in, allowing light in and out of the otherwise sealed DSPC. The sample itself is introduced through the top of the DSPC manually (frequently, using a pipette) or with the OLIS Automatic Titrator(quoted separately).
In addition, a water jacketed cell holder for a normal 1 cm2 cuvette is included. This opens the use of the spectrophotometer/ fluorimeter for those times when the pathlength enhancement and/or scatter immunity of the DSPC is not needed or appropriate. The two sample holder assembles are quickly exchanged one for the other. Order water bath separately.
The Windoes 10 compatible SpectralWorks software is provided for all instrument control, data acquisition, data handling, and data fitting. Fitting algorithms include those of the Matheson-DeSa GlobalWorks suite, plus many for 2D analysis including peak finder, spectral deconvolution, difference spectra, derivative, integration, etc. Data storage and retrieval, with import/ export capabilities for ASCII and EXCEL file types are provided for dealing with OLIS and non-OLIS acquired data. The provided computer hardware is an OLIS verified and supported desktop PC with 3rd Gen. Intel Core i3-3240 3.4GHz, 4GB DDR3, 1TB HDD 7,200RPM, DVD-RW drive, 6 Total USB 2.0 Ports. Windows 10 Pro 64-bit (English version required), Keyboard & Mouse and 20" Class Monitor.
Software supports data acquisition and handling modes for scanning excitation, fixed wavelength excitation, scanning emission, fixed wavelength emission, synchronous scanning of emission and excitation, light scatter, and single beam absorbance of data in 2D and 3D. Base of the instrument dimensions are approximately 22" x 29".
OLIS CLARiTY 245
这是一款研究级别的扫描荧光光谱仪，配备有一台OLIS双光栅f/4.2 Hummingbird单色器，位于激发侧，并且有一台OLIS单光栅“姐妹”单色器，位于发射侧。激发光源是一个水冷却的150瓦氙气弧灯，安装在镜面椭圆灯罩内，设计用于提供最大强度。灯通过高精度调节电源点火和运行，以实现高度可靠的点火、超低波纹和高精度电流调节。支持计算机化的发射和激发波长选择，软件可设置扫描和信号平均。检测速率高达10毫秒，可在采集期间或之后进行任意数量的信号平均，以优化速度或最佳灵敏度。提供一个数字光子计数模块（PCM），用于从230-870纳米的极其敏感的发射读数。第二个探测器是高精度模拟光电倍增管，用于吸收读数，直接安装在样品旁边。
样品室是一个单光束的CLARiTY室。这个“CLARiTY”室消除了散射的影响，因此可以对混浊样品悬浮液进行准确的吸收和荧光光谱分析。在“CLARiTY”室内是“悬浮呈现腔体”（DSPC），它可以是高吸收（高达22 AU）的微型比色皿，或者是1毫升或8毫升（低吸收，到纳摩尔浓度）石英样品架。分别返回有效路径长度为0.25毫米、5厘米或30厘米。当样品容量不受限制时，较大的DSPC是最好的选择，例如E.coli、酵母、藻类和所有其他丰富和/或廉价的样品。大容量的好处是大约30厘米的巨大路径长度增强。较小的DSPC允许使用有限的样品体积（例如胚胎斑马鱼或婴儿血细胞）。路径长度增强要少得多，对于1毫升体积只有4厘米。但是，使用相同的光强度灌注了更小的体积，因此检测到的信号更强，因此信噪比更高。内置了持有石英棒的端口，允许光线进出否则密封的DSPC。样品本身是通过DSPC顶部手动引入的（经常使用移液管）或通过OLIS自动滴定器（单独报价）。
此外，还包括一个适用于普通1平方厘米比色皿的水夹套细胞支架。这使得光谱仪/荧光仪可以在不需要或不适合DSPC的情况下使用，比如路径长度增强和/或散射免疫时。两种样品支架组件可以快速互换。水浴单独订购。
提供与Windows 10兼容的SpectralWorks软件，用于仪器控制、数据采集、数据处理和数据拟合。拟合算法包括Matheson-DeSa GlobalWorks套件的算法，以及许多用于二维分析的算法，包括峰值查找器、光谱分解、差异光谱、导数、积分等。提供数据存储和检索功能，并支持ASCII和EXCEL文件类型的导入/导出，用于处理OLIS和非OLIS获取的数据。提供的计算机硬件是经过OLIS验证和支持的台式PC，配备第三代英特尔Core i3-3240 3.4GHz、4GB DDR3、1TB HDD 7,200RPM、DVD-RW驱动器、总共6个USB 2.0端口。Windows 10 Pro 64位（需要英文版本）、键盘和鼠标以及20英寸显示器。
该软件支持扫描激发、固定波长激发、扫描发射、固定波长发射、发射和激发同步扫描、光散射以及二维和三维数据的单光束吸收模式的数据采集和处理。仪器的底座尺寸约为22英寸 x 29英寸。
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