Olis, Inc Olis OxyDye Oxygen Measurement System 测氧仪
This enhancement is suitable for use with in a wide range of spectrophotometers and
fluorimeters and is not limited to use with Olis equipment. The oxygen reading is synchronized
to be non-interfering with other optical measurements on-going by the primary data analysis
program.
The OxyDye method uses phosphorescence lifetime as a measure of oxygen concentration. A
submicromolar concentration of dye (harmless to biological materials) is added to the given
sample (dye purchased separately). A pulsed LED excites dye in the sample to luminescence and
a PMT used as a photon counter detects the emitted light. The counts from the detector are collected by 100 digital counters on an FPGA card. The decay of the luminesce (its lifetime)
varies from about 5 to 60 microseconds depending on the oxygen concentration. The LED
excitation pulses can be repeated at rates to 5000 per second, so that only considerations of the
S/N required in a particular experiment limits the response time of the system to the oxygen
concentration. The acquired 100 points of the decay profile are read from the counters and are
fitted to one exponential; the resulting decay constant (Tau) is converted to oxygen concentration
using the Stern-Volmer equation. The measurement is unaffected by fluorescence and the
intensity of the phosphorescence.
The OxyDye enhancement to an Olis spectrophotometer includes the LED, fiber optics, photon
counting module, supporting electronics, and software enhancement.
Olis, Inc Olis OxyDye Oxygen Measurement System 测氧仪
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