Olis, Inc Phosphorescence Lifetime Enhancement to OLIS CPL Spectrometer PLE-OLIS-CPL-210114
This enhancement employs the electronics and detector from the OLIS CPL spectrometer and adds the required hardware and software for adding phosphorescence lifetime measurement. With this enhancement, one will know whether s/he is measuring circularly polarized luminescence (CPL) or circularly polarized phosphorescence (CPP). The add-on will include an interchangeable LED installed in a PMT mount and the host fluorimeter photon counter control box will be modified for LED communication. The OLIS PCPH software package is provided for controlling LED.
The OLIS method uses phosphorescence lifetime to measure submicromolar concentration of dye (harmless to biological materials) added to the given sample (dye purchased separately). A pulsed LED excites dye in the sample to luminesce and a PMT used as a photon counter detects the emitted light. The counts from the detector are collected by 100 digital counters on an FPGA card. The decay of the luminescence (its lifetime) varies from about 5 to 60 microseconds. The LED excitation pulses can be repeated at rates to 5000 per second, so that only considerations of the S/N require in a particular experiment limits the response time of the system. The acquired 100 points of the decay profile are read from the counters and can be fitted to one exponential. The measurement is unaffected by fluorescence and the intensity of the phosphorescence.
Olis, Inc Phosphorescence Lifetime Enhancement to OLIS CPL Spectrometer PLE-OLIS-CPL-210114
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